Medical Laboratory Technician MCQ Quiz - Objective Question with Answer for Medical Laboratory Technician - Download Free PDF

Last updated on Apr 11, 2025

Latest Medical Laboratory Technician MCQ Objective Questions

Medical Laboratory Technician Question 1:

Which of the following is the most effective method for preventing hepatitis B infection?

  1. Safe drinking water
  2. Use of clean needles and vaccination
  3. Wearing protective clothing
  4. Avoiding sunlight exposure

Answer (Detailed Solution Below)

Option 2 : Use of clean needles and vaccination

Medical Laboratory Technician Question 1 Detailed Solution

Correct Answer: Use of clean needles and vaccination
Rationale:
  • Hepatitis B is a viral infection that affects the liver, caused by the hepatitis B virus (HBV). It can lead to chronic liver disease, liver cancer, and death if not prevented or treated properly.
  • The most effective method for preventing hepatitis B infection is through vaccination. The hepatitis B vaccine is highly effective in providing immunity against the virus. It is usually given in a series of three or four shots over six months.
  • Using clean needles is crucial, particularly for individuals who inject drugs. Sharing needles can spread HBV, so using sterile, single-use needles can greatly reduce the risk of infection.
  • Both strategies, vaccination and the use of clean needles, address the primary modes of HBV transmission: percutaneous (through the skin) and mucosal exposure to infectious blood or body fluids.
Explanation of Other Options:
Safe drinking water
  • Rationale: While safe drinking water is essential for overall health and preventing waterborne diseases, it does not play a significant role in preventing hepatitis B infection, which is primarily spread through blood and body fluids.
Wearing protective clothing
  • Rationale: Wearing protective clothing can help prevent infections in healthcare settings or in environments with a high risk of exposure to bloodborne pathogens. However, it is not a primary method for preventing hepatitis B infection among the general population.
Avoiding sunlight exposure
  • Rationale: Avoiding sunlight exposure is not related to the prevention of hepatitis B. Sunlight exposure is more relevant to preventing skin cancers and other conditions caused by UV radiation.
Conclusion:
  • Among the given options, the use of clean needles and vaccination is the most effective method for preventing hepatitis B infection. These methods directly target the routes of transmission and provide immunity, significantly reducing the risk of infection.

Medical Laboratory Technician Question 2:

In healthcare settings, what role does interdepartmental communication play in patient care?

  1. Prevents necessary information from being shared
  2. Ensures that all departments work independently without collaboration
  3. Enhances the coordination between different healthcare professionals, leading to better patient outcomes
  4. Limits patient access to healthcare services

Answer (Detailed Solution Below)

Option 3 : Enhances the coordination between different healthcare professionals, leading to better patient outcomes

Medical Laboratory Technician Question 2 Detailed Solution

Correct Answer: Enhances the coordination between different healthcare professionals, leading to better patient outcomes
Rationale:
  • Interdepartmental communication is essential in healthcare settings as it ensures that all relevant information about a patient's condition, treatment, and progress is shared among the different departments involved in the patient's care.
  • Effective communication enhances the coordination between different healthcare professionals, allowing for a more cohesive and comprehensive approach to patient care.
  • When departments communicate effectively, it reduces the chances of errors, ensures that treatments and interventions are consistent, and enables timely decision-making, all of which contribute to better patient outcomes.
  • Additionally, interdepartmental communication fosters a collaborative environment where healthcare professionals can share their expertise and insights, leading to more accurate diagnoses and effective treatment plans.
Explanation of Other Options:
Prevents necessary information from being shared
  • Rationale: This option is incorrect because effective interdepartmental communication actually facilitates the sharing of necessary information, rather than preventing it.
Ensures that all departments work independently without collaboration
  • Rationale: This option is incorrect because the goal of interdepartmental communication is to promote collaboration, not independence. Collaboration is key to providing comprehensive care.
Limits patient access to healthcare services
  • Rationale: This option is incorrect because effective communication does not limit patient access to healthcare services. Instead, it ensures that patients receive timely and appropriate care by coordinating efforts across departments.
Conclusion:
  • In summary, interdepartmental communication plays a crucial role in enhancing coordination among healthcare professionals, leading to better patient outcomes. It ensures that information is shared, collaboration is promoted, and patient care is optimized.

Medical Laboratory Technician Question 3:

Which of the following is an important aspect of effective staff selection in healthcare organizations?

  1. Personal connections
  2. Relevant skills and experience
  3. Appearance alone
  4. Age of the candidate

Answer (Detailed Solution Below)

Option 2 : Relevant skills and experience

Medical Laboratory Technician Question 3 Detailed Solution

Correct Answer: Relevant skills and experience
Rationale:
  • Effective staff selection in healthcare organizations is crucial for ensuring high-quality patient care, operational efficiency, and overall organizational success. One of the most important aspects of this process is evaluating candidates based on their relevant skills and experience.
  • Relevant skills and experience ensure that the selected candidates have the necessary knowledge, competencies, and practical know-how to perform their job responsibilities effectively. This includes clinical skills, familiarity with healthcare protocols, and the ability to handle patient care tasks efficiently.
  • Experience in healthcare settings is particularly valuable as it demonstrates that the candidate has been exposed to real-world scenarios and has gained practical insights that can enhance their performance in the new role. It also indicates that the candidate is likely to adapt quickly to the new work environment.
Explanation of Other Options:
Personal connections
  • Rationale: While personal connections may sometimes help in getting a foot in the door, they should not be the primary criterion for staff selection. Hiring based on personal connections can lead to favoritism and may not necessarily result in selecting the most qualified candidate for the job.
Appearance alone
  • Rationale: Appearance alone is not a valid criterion for staff selection in healthcare. While maintaining a professional appearance is important, it does not reflect a candidate's ability to perform job duties, their skills, or their experience. Healthcare roles require substantial competence, which appearance cannot indicate.
Age of the candidate
  • Rationale: Age should not be a determining factor in the selection process. Focusing on age can lead to age discrimination and does not necessarily correlate with the candidate's ability to perform effectively in their role. Skills, experience, and qualifications are more important indicators of a candidate's suitability for the job.
Conclusion:
  • Among the given options, relevant skills and experience are the most critical aspects of effective staff selection in healthcare organizations. This ensures that the candidates can deliver high-quality care, adapt to the work environment, and contribute positively to the organization's goals. Other factors like personal connections, appearance alone, and age are not reliable indicators of a candidate's ability to perform in healthcare roles.

Medical Laboratory Technician Question 4:

What is the primary focus of public relations in a healthcare setting?

  1. Establishing and maintaining positive interactions with patients and the community
  2. Focusing on the financial outcomes of healthcare institutions 
  3. Focusing on administrative paperwork
  4. Limiting communication between staff and patients

Answer (Detailed Solution Below)

Option 1 : Establishing and maintaining positive interactions with patients and the community

Medical Laboratory Technician Question 4 Detailed Solution

Correct Answer: Establishing and maintaining positive interactions with patients and the community
Rationale:
  • The primary focus of public relations in a healthcare setting is to establish and maintain positive interactions with patients and the community. This involves creating trust and a positive image of the healthcare institution, ensuring that the services provided are well communicated, and fostering a sense of community engagement.
  • Effective public relations activities can improve patient satisfaction, enhance the reputation of the healthcare institution, and facilitate better communication between the healthcare providers and the community they serve.
  • Key activities include managing media relations, organizing community events, handling patient feedback, and ensuring transparent communication about healthcare services, policies, and changes.
Explanation of Other Options:
Focusing on the financial outcomes of healthcare institutions
  • Rationale: While financial outcomes are important for the sustainability of healthcare institutions, public relations is more concerned with communication and relationship-building rather than directly focusing on financial metrics.
Focusing on administrative paperwork
  • Rationale: Administrative paperwork is typically handled by administrative staff and is not the focus of public relations. Public relations professionals are more involved in managing communication strategies and enhancing the institution's image.
Limiting communication between staff and patients
  • Rationale: Limiting communication is contrary to the goals of public relations. Instead, public relations aims to facilitate open and effective communication between staff and patients to build trust and improve service quality.
Conclusion:
  • The correct answer highlights the core objective of public relations in healthcare, which is to build and maintain positive relationships and effective communication with patients and the community. This is crucial for ensuring patient satisfaction, enhancing the institution’s reputation, and fostering community trust.

Medical Laboratory Technician Question 5:

Effective communication between healthcare teams is essential for:

  1. Reducing administrative workload
  2. Ensuring patient safety and improving care coordination
  3. Focusing on the hospital's profit margins
  4. Fostering interdepartmental conflicts

Answer (Detailed Solution Below)

Option 2 : Ensuring patient safety and improving care coordination

Medical Laboratory Technician Question 5 Detailed Solution

Correct Answer: Ensuring patient safety and improving care coordination
Rationale:
  • Effective communication between healthcare teams is critical for ensuring patient safety and improving care coordination. When healthcare providers communicate clearly and effectively, they can share important patient information, discuss treatment plans, and coordinate care activities to avoid errors and ensure that patients receive the best possible care.
  • Good communication helps in the timely and accurate exchange of information, which is essential for making informed decisions about patient care. It helps in the reduction of medical errors, improves patient outcomes, and enhances overall patient satisfaction.
Explanation of Other Options:
Reducing administrative workload
  • Rationale: While effective communication can indirectly help in reducing administrative workload by preventing errors and streamlining processes, the primary goal is to ensure patient safety and improve care coordination.
Focusing on the hospital's profit margins
  • Rationale: Effective communication between healthcare teams is not primarily aimed at focusing on the hospital's profit margins. The main objective is to improve patient care. Financial considerations are important but secondary to patient safety and care quality.
Fostering interdepartmental conflicts
  • Rationale: Fostering interdepartmental conflicts is not a goal of effective communication. In fact, effective communication aims to reduce conflicts by promoting understanding and collaboration among different departments within the healthcare facility.
Conclusion:
  • Effective communication is essential in healthcare settings to ensure patient safety and improve care coordination. It helps in the timely exchange of critical information, reduces the risk of errors, and enhances patient outcomes. Other options, such as reducing administrative workload, focusing on profit margins, or fostering conflicts, are not the primary goals of effective communication in healthcare.

Top Medical Laboratory Technician MCQ Objective Questions

Medical Laboratory Technician Question 6:

Which immunoglobulin can cross the placenta?

  1. IgG
  2. IgM
  3. IgA
  4. IgE

Answer (Detailed Solution Below)

Option 1 : IgG

Medical Laboratory Technician Question 6 Detailed Solution

Correct Answer: IgG
Rationale:
  • Immunoglobulin G (IgG) is the only type of immunoglobulin that can cross the placenta from the mother to the fetus. This transfer provides passive immunity to the fetus, protecting it from infections during the early stages of life until the newborn's immune system becomes fully functional.
  • IgG is the most abundant type of antibody in the blood and extracellular fluid, allowing it to control infection of body tissues. By crossing the placenta, IgG helps in protecting the fetus against bacterial and viral infections.
Explanation of Other Options:
IgM
  • Rationale: Immunoglobulin M (IgM) is the first antibody produced in response to an infection. However, IgM is a larger molecule compared to IgG and does not cross the placenta. Its primary role is to eliminate pathogens in the early stages of immune response before there is sufficient IgG.
IgA
  • Rationale: Immunoglobulin A (IgA) is primarily found in mucous membranes lining the respiratory and gastrointestinal tracts. It plays a crucial role in mucosal immunity but does not cross the placenta. IgA is also present in breast milk, providing important immune protection to breastfeeding infants.
IgE
  • Rationale: Immunoglobulin E (IgE) is associated with allergic reactions and defense against parasitic infections. It binds to allergens and triggers histamine release from mast cells and basophils, but it does not cross the placenta.
Conclusion:
  • Among the given options, IgG is the only immunoglobulin that can cross the placenta, providing essential passive immunity to the fetus. The other immunoglobulins (IgM, IgA, and IgE) serve specific roles in the immune system but are not capable of crossing the placental barrier.

Medical Laboratory Technician Question 7:

What could cause urine specimens to appear cloudy?

  1. Bacteria
  2. Red blood cells (RBC)
  3. Pus cells
  4. All of the above

Answer (Detailed Solution Below)

Option 4 : All of the above

Medical Laboratory Technician Question 7 Detailed Solution

Correct Answer: All of the above
Rationale:
  • Cloudy urine can be caused by the presence of various substances, including bacteria, red blood cells (RBCs), and pus cells. These substances can indicate different underlying conditions or infections.
  • Bacteria in the urine can cause it to appear cloudy. This is often a sign of a urinary tract infection (UTI), where the presence of bacteria leads to an inflammatory response in the urinary tract.
  • Red blood cells (RBCs) can also cause urine to appear cloudy. Hematuria, or the presence of blood in the urine, can result from various conditions, including kidney stones, infections, or trauma to the urinary system.
  • Pus cells, or white blood cells (WBCs), in the urine are another potential cause of cloudiness. This is often associated with infections or inflammations of the urinary tract, where the body's immune response leads to an increased presence of WBCs in the urine.
Explanation of Other Options:
Bacteria
  • Rationale: While bacteria can indeed cause cloudy urine, it is not the sole cause. Bacterial presence typically indicates a urinary tract infection (UTI), but other substances can also contribute to urine cloudiness.
Red blood cells (RBC)
  • Rationale: RBCs can make urine appear cloudy, often due to conditions like hematuria. However, the presence of RBCs alone is not the only reason for cloudy urine, as other cells and substances can also contribute to this appearance.
Pus cells
  • Rationale: Pus cells can cause urine cloudiness, indicating an infection or inflammation. However, similar to bacteria and RBCs, pus cells are not the sole cause of cloudy urine.
Conclusion:
  • All of the above options (bacteria, red blood cells, and pus cells) can individually or collectively cause urine to appear cloudy. Therefore, the most comprehensive answer is "All of the above," recognizing that multiple factors can contribute to the cloudiness of urine.

Medical Laboratory Technician Question 8:

What is the principle behind the reagent strip test for detecting blood?

  1. Binding of heme and chromogenic dye
  2. Peroxidase activity of heme
  3. Reaction of peroxidase and chromogen
  4. Diazo activity of heme

Answer (Detailed Solution Below)

Option 2 : Peroxidase activity of heme

Medical Laboratory Technician Question 8 Detailed Solution

Correct Answer: Peroxidase activity of heme
Rationale:
  • The principle behind the reagent strip test for detecting blood is based on the peroxidase activity of heme, which is a component of hemoglobin in red blood cells.
  • Heme acts as a pseudo-peroxidase. In the presence of hydrogen peroxide, heme catalyzes the oxidation of a chromogen on the reagent strip, causing a color change.
  • The appearance of a color on the strip indicates the presence of blood, as the heme in red blood cells or myoglobin in muscle cells is responsible for this reaction.
Explanation of Other Options:
Binding of heme and chromogenic dye
  • Rationale: This option is incorrect because the test does not rely on the binding of heme to a chromogenic dye. Instead, the test is based on the catalytic activity of heme in the presence of hydrogen peroxide and a chromogen.
Reaction of peroxidase and chromogen
  • Rationale: Although this option is somewhat related, it is not entirely correct. The test specifically relies on the peroxidase activity of heme, not just any peroxidase. The reaction involves heme acting as a pseudo-peroxidase.
Diazo activity of heme
  • Rationale: This option is incorrect because the reagent strip test for detecting blood does not involve diazo activity. Diazo reactions are typically used for detecting other substances, such as bilirubin, not blood.
Conclusion:
  • The principle behind the reagent strip test for detecting blood is the peroxidase activity of heme. This activity causes a color change on the test strip in the presence of hydrogen peroxide and a chromogen, indicating the presence of blood. Other options are incorrect as they do not accurately describe the mechanism of the test.

Medical Laboratory Technician Question 9:

Which type of cells develop from yolk sacs during the mesoblastic phase?

  1. Portland hemoglobin
  2. Gower 1 and Gower 2 hemoglobin
  3. Hb F, Hb A2, and Hb A
  4. Only erythroblasts

Answer (Detailed Solution Below)

Option 2 : Gower 1 and Gower 2 hemoglobin

Medical Laboratory Technician Question 9 Detailed Solution

Correct Answer: Gower 1 and Gower 2 hemoglobin
Rationale:
  • During the mesoblastic phase of embryonic development, which occurs in the early stages of gestation, the yolk sac is the primary site of hematopoiesis (blood cell formation). It is within the yolk sac that primitive erythroblasts are formed, which subsequently give rise to primitive hemoglobin types.
  • The primary types of hemoglobin produced during this phase are Gower 1 and Gower 2 hemoglobin. These embryonic hemoglobins are essential for the efficient transport of oxygen to the developing embryo.
  • Gower 1 hemoglobin consists of zeta and epsilon chains, while Gower 2 hemoglobin is composed of alpha and epsilon chains. These types of hemoglobin are unique to the early stages of development and are replaced by fetal and adult hemoglobin as development progresses.
Explanation of Other Options:
Portland hemoglobin
  • Rationale: Portland hemoglobin is another type of embryonic hemoglobin, composed of zeta and gamma chains. It is also present during the early stages of development, but Gower 1 and Gower 2 are more predominant in the yolk sac during the mesoblastic phase.
Hb F, Hb A2, and Hb A
  • Rationale: Hb F (fetal hemoglobin), Hb A2, and Hb A are forms of hemoglobin that appear later in development. Hb F is the predominant form of hemoglobin during fetal development, while Hb A becomes the dominant form after birth. These types are not produced during the mesoblastic phase in the yolk sac.
Only erythroblasts
  • Rationale: While erythroblasts are indeed the precursors to red blood cells and are formed in the yolk sac during the mesoblastic phase, the question specifically asks about the types of hemoglobin produced. Gower 1 and Gower 2 hemoglobin are the correct types associated with this developmental stage.
Conclusion:
  • During the mesoblastic phase, the yolk sac is responsible for the production of primitive erythroblasts, which give rise to Gower 1 and Gower 2 hemoglobin. These types of hemoglobin are essential for the early oxygen transport needs of the developing embryo and are distinct from the hemoglobin types that appear later in fetal development.

Medical Laboratory Technician Question 10:

Which Red Cell Index evaluates RBC saturation with hemoglobin (Hb) in the range of 32–36%?

  1. MCHC
  2. MCV
  3. MCH
  4. None

Answer (Detailed Solution Below)

Option 1 : MCHC

Medical Laboratory Technician Question 10 Detailed Solution

Correct Answer: MCHC
Rationale:
  • Mean Corpuscular Hemoglobin Concentration (MCHC) is the Red Cell Index that evaluates the concentration of hemoglobin (Hb) in a given volume of packed red blood cells. It reflects the average saturation of hemoglobin in the red blood cells and is expressed in the range of 32–36%.
  • MCHC provides information about the color and hemoglobin content of red blood cells. It is useful in diagnosing and classifying anemias.
  • A normal MCHC value indicates that the red blood cells have an adequate amount of hemoglobin, while low or high values can indicate hypochromic or hyperchromic anemias, respectively.
Explanation of Other Options:
MCV
  • Rationale: Mean Corpuscular Volume (MCV) measures the average volume or size of individual red blood cells. It is expressed in femtoliters (fL). MCV helps in differentiating the types of anemia based on the size of red blood cells, such as microcytic, normocytic, and macrocytic anemias.
MCH
  • Rationale: Mean Corpuscular Hemoglobin (MCH) indicates the average amount of hemoglobin per red blood cell. It is calculated by dividing the total hemoglobin by the red blood cell count and is expressed in picograms (pg). MCH helps in identifying the hemoglobin content in red blood cells, but it does not evaluate the concentration or saturation of hemoglobin within the cells.
None
  • Rationale: This option is incorrect because MCHC is indeed the correct Red Cell Index that evaluates RBC saturation with hemoglobin in the range of 32–36%.
Conclusion:
  • Among the given options, MCHC is the index that specifically measures the concentration of hemoglobin in red blood cells, providing valuable information about their hemoglobin saturation. This index is crucial in diagnosing various types of anemia and understanding the hemoglobin content in red blood cells.

Medical Laboratory Technician Question 11:

What is the main difference between serum and plasma?

  1. Serum contains cells, whereas plasma is clear and yellow.
  2. Plasma has more antibodies than serum.
  3. Serum is colorless, while plasma is yellow.
  4. Plasma contains coagulation factors, whereas serum does not.

Answer (Detailed Solution Below)

Option 4 : Plasma contains coagulation factors, whereas serum does not.

Medical Laboratory Technician Question 11 Detailed Solution

Correct Answer: Plasma contains coagulation factors, whereas serum does not.
Rationale:
  • The primary difference between serum and plasma is the presence of coagulation factors. Plasma is the liquid component of blood that holds blood cells in suspension. It contains water, electrolytes, proteins, hormones, and waste products. Importantly, plasma includes coagulation factors that are essential for blood clotting.
  • Serum, on the other hand, is the fluid that remains after blood has clotted. During the clotting process, the coagulation factors are consumed and removed from the liquid portion, leaving behind serum, which is devoid of these factors.
  • The distinction is crucial in medical diagnostics and treatment, as plasma is used for coagulation studies and transfusions to manage bleeding disorders, whereas serum is often used for various types of chemical and immunological tests.
Explanation of Other Options:
Serum contains cells, whereas plasma is clear and yellow.
  • Rationale: This statement is incorrect because neither serum nor plasma contains blood cells. Both are cell-free components of blood, though plasma is typically clear and yellow due to the presence of proteins and other substances, while serum can be similarly colored but is free of clotting factors.
Plasma has more antibodies than serum.
  • Rationale: This statement is not entirely accurate. Both plasma and serum contain antibodies. However, the concentration of antibodies in serum can be higher, particularly after an immune response, because it lacks the coagulation factors present in plasma.
Serum is colorless, while plasma is yellow.
  • Rationale: This statement is incorrect. Both serum and plasma are typically clear and yellow. The yellow color is due to the presence of bilirubin and other substances. The main difference is the absence of clotting factors in serum.
Conclusion:
  • In summary, the main difference between serum and plasma lies in the presence of coagulation factors. Plasma contains these factors and is used for coagulation-related studies and treatments. Serum, which lacks coagulation factors, is primarily used in chemical and immunological testing. Understanding this distinction is critical in both clinical and laboratory settings.

Medical Laboratory Technician Question 12:

During acute inflammation, which cells will produce predominant response?

  1. Lymphocyte
  2. Macrophages
  3. Neutrophil
  4. Mast cells

Answer (Detailed Solution Below)

Option 3 : Neutrophil

Medical Laboratory Technician Question 12 Detailed Solution

Correct Answer: Neutrophil
Rationale:
  • Acute inflammation is the body's immediate response to tissue injury or infection. It involves a series of complex biological processes aimed at eliminating the initial cause of cell injury, clearing out necrotic cells and tissues, and establishing a repair environment.
  • Neutrophils are the predominant cells during the acute inflammatory response. They are a type of white blood cell that rapidly migrate to the site of inflammation within minutes to hours of the injury or infection.
  • Neutrophils function by phagocytosing (engulfing) and digesting pathogens and debris through the release of enzymes and reactive oxygen species.
Explanation of Other Options:
Lymphocytes
  • Rationale: Lymphocytes are a key component of the adaptive immune response, not the acute inflammatory response. They are primarily involved in the later stages of inflammation and immune response, including the recognition of specific antigens and the production of antibodies.
Macrophages
  • Rationale: Macrophages play a significant role in both acute and chronic inflammation. However, they are not as rapidly mobilized as neutrophils. Macrophages arrive at the site of inflammation later and are involved in phagocytosis, antigen presentation, and cytokine production to regulate the immune response.
Mast Cells
  • Rationale: Mast cells are involved in the early stages of inflammation, particularly in allergic reactions. They release histamine and other mediators that contribute to vasodilation and increased vascular permeability. However, they are not the predominant cell type during acute inflammation.
Conclusion:
  • Among the given options, neutrophils are the primary cells involved in the acute inflammatory response. Their rapid recruitment and ability to phagocytose pathogens make them crucial for the initial defense against infection and tissue injury.
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Medical Laboratory Technician Question 13:

Which reagent is not used to concentrate and stain ova and parasite specimens?

  1. Zinc Sulfate
  2. Crystal Violet
  3. 10% Formalin
  4. Ether

Answer (Detailed Solution Below)

Option 2 : Crystal Violet

Medical Laboratory Technician Question 13 Detailed Solution

The correct option is:

2) Crystal Violet

Explanation:

  • Zinc Sulfate: This reagent is commonly used for concentrating ova and parasite specimens by creating a flotation method, where the parasitic organisms float to the top of the solution.
  • 10% Formalin: This is typically used to preserve stool samples and also to concentrate ova and parasites in parasitology.
  • Ether: Ether is used in a concentration technique for stool specimens, particularly for the sedimentation method, where it helps separate the parasites from the stool sample.
  • Crystal Violet: This is a dye used in microbiological staining (e.g., Gram staining), but it is not used for concentrating or staining ova and parasite specimens.

Additional Information:

Concentration and staining techniques are important for detecting parasites in stool samples. Zinc sulfate, 10% formalin, and ether are commonly used for these purposes, but crystal violet is not used in parasitology for this specific purpose.

Medical Laboratory Technician Question 14:

Giemsa stain is particularly useful in identifying:

  1. Parasitic infections
  2. Viral inclusions
  3. Bacterial infections
  4. Fungal infections

Answer (Detailed Solution Below)

Option 1 : Parasitic infections

Medical Laboratory Technician Question 14 Detailed Solution

Correct Answer: Parasitic infections
Rationale:
  • Giemsa stain is a widely used differential stain that is particularly effective in identifying and diagnosing parasitic infections, including malaria and other protozoan infections.
  • This stain works by binding to the DNA and RNA of parasites, making them visible under a microscope. The Giemsa stain technique highlights the internal structures of the parasites, including their nuclei and cytoplasmic inclusions, allowing for clear identification.
  • It is especially useful in blood smears, where it can detect the presence of Plasmodium species, the causative agents of malaria, by staining their distinctive ring forms and other stages within red blood cells.
Explanation of Other Options:
Viral inclusions
  • Rationale: Giemsa stain is not typically used for identifying viral inclusions. Viral inclusions are better visualized with other staining techniques such as immunohistochemistry or electron microscopy, which are more suited for detecting viral particles and associated cellular changes.
Bacterial infections
  • Rationale: While Giemsa stain can be used to visualize some bacteria, it is not the most specific or commonly used stain for bacterial identification. Gram stain is the preferred method for classifying and identifying bacteria based on their cell wall characteristics.
Fungal infections
  • Rationale: Fungal infections are typically identified using special stains such as Periodic Acid-Schiff (PAS) or Gomori methenamine silver (GMS) stains, which are more effective in highlighting fungal elements like hyphae and spores in tissue samples.
Conclusion:
  • Among the given options, Giemsa stain is particularly useful for identifying parasitic infections due to its effectiveness in staining and highlighting the structures of parasites, especially in blood smears. It is not the preferred staining method for viral, bacterial, or fungal infections, which require different specific staining techniques.
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Medical Laboratory Technician Question 15:

What is the main function of a fixative?

  1. To remove water from tissues
  2. To preserve tissue morphology and prevent autolysis
  3. To stain tissue
  4. To harden tissues for sectioning

Answer (Detailed Solution Below)

Option 2 : To preserve tissue morphology and prevent autolysis

Medical Laboratory Technician Question 15 Detailed Solution

Correct Answer: To preserve tissue morphology and prevent autolysis
Rationale:
  • The main function of a fixative in histology is to preserve tissue morphology and prevent autolysis. Fixatives work by stabilizing the structure of tissues and cells, making them resistant to decay and decomposition. This is crucial for accurate histological examination and diagnosis.
  • By cross-linking proteins and nucleic acids, fixatives help to maintain the architecture of the tissue, thus allowing for detailed and precise microscopic analysis. This preservation is essential in both clinical and research settings to ensure the reliability of the results.
Explanation of Other Options:
To remove water from tissues
  • Rationale: Removing water from tissues is not the primary function of a fixative. Dehydration is typically achieved through a series of graded alcohols during tissue processing, not during fixation.
To stain tissue
  • Rationale: Staining tissue is a separate step that usually occurs after fixation. Stains are used to provide contrast and highlight specific structures within the tissue, but they do not preserve or stabilize the tissue structure.
To harden tissues for sectioning
  • Rationale: While some fixatives may harden tissues, this is not their main function. Hardening is a side effect that can aid in the subsequent cutting of thin sections, but the primary goal of fixation is to preserve tissue morphology and prevent autolysis.
Conclusion:
  • Among the given options, the primary role of a fixative is to preserve tissue morphology and prevent autolysis. This preservation is crucial for enabling detailed and accurate examination of tissues under the microscope, which is essential for both diagnostic and research purposes.
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